Use of an Optimized Transient Occlusion of the Middle Cerebral Artery Protocol for the Mouse Stroke Model

Intraluminal occlusion of the middle cerebral artery in rodents is widely used for investigating cerebral ischemia and reperfusion injury. Two types of filaments used for occlusion were tested in terms of surgical success, incidence of subarachnoid hemorrhage, and mortality: a standard 6-0 monofilament coated with methyl methacrylate glue (rigid probe) and an 8-0 monofilament coated with silicone (flexible probe). In 98 wild-type (WT) mice, the flexible probe produced significantly (P < .05) more successful strokes (73.5%) than the rigid probe (46.6%). The incidences of subarachnoid hemorrhage (3.7%) and mortality (5.6%) with the flexible probe were significantly lower than those with the rigid probe (26.6% and 11.1%, respectively). Rigid and flexible probes were also compared in heme oxygenase 1 knockout (n = 17) and WT littermates (n = 17), because knockout mice have been suggested to have more fragile blood vessels. All mice receiving the flexible probe had successful strokes, with no cases of subarachnoid hemorrhage or mortality; however, with the rigid probe, the success rate was only 80% in the WT mice and 60% in the knockout mice. The rates of subarachnoid hemorrhage and mortality were also significantly higher with the rigid probe in both genotypes, but the infarct volumes produced by each type of probe did not differ significantly between the 2 groups. We conclude that the flexible silicone-coated 8-0 probe is superior to the more rigid glue-coated probe, because it produces infarct volumes of equal size with a higher success rate and lower risk of subarachnoid hemorrhage and mortality.

Key Words:  Infarction , ischemia , middle cerebral artery occlusion , silicone filament