The purpose of this study was to evaluate the effect of intravenous infusion of basic
fibroblast growth factor (bFGF) in a permanent ischemia model at the subacute phase
(2 weeks) as well as at 24 hours and 1 week using T2-weighted magnetic resonance imaging (MRI). The middle cerebral artery (MCA) in Sprague-Dawley
rats was occluded using an intraluminal suture method. The rats were randomly divided
into 2 groups to receive either bFGF (45 μg/kg/hr) or saline solution. The infusion
was started 30 minutes after MCA occlusion (MCAO) and continued for 3 hours. Regional
cerebral blood flow (rCBF) was measured using laser Doppler flowmetry throughout the
infusion. T2-weighted MRI was carried out before MCAO, 24 hours after MCAO, and days 7 and 14
after MCAO. Although an elevation in rCBF was seen after the infusion, no significant
change between the groups was observed. A significant difference between the bFGF
and saline groups in T2-derived lesion volume was observed at 24 hours (P < .05), on day 7 (P < .05), and on day 14 (P < .01). The percentage of lesion area calculated from the ipsilateral hemisphere
using hematoxylin and eosin staining on day 14 showed a significant difference between
the bFGF and saline groups (P < .05). No significant change in the number of bromodeoxyuridine (BrdU)-labeled cells
between the groups was observed. This study demonstrates that bFGF, infused intravenously
starting 30 minutes after the induction of permanent MCAO, significantly reduces region
volume even at day 14, as well as at days 1 and 7, compared with the corresponding
saline group.
Key Words
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Article info
Publication history
Accepted:
May 10,
2005
Received in revised form:
March 30,
2005
Received:
December 29,
2004
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2005.05.004
Copyright
© 2005 National Stroke Association. Published by Elsevier Inc. All rights reserved.
