Background
Few studies have examined the relationship between mircroRNAs and moyamoya disease
(MMD). We performed a study of the significance of let-7c expression in the serum
of MMD patients.
Methods
The experimental group includes 49 MMD patients, and the control group consists of
30 normal people, 20 cerebral hemorrhage patients, 20 massive cerebral infarction
patients, 20 nonmassive cerebral infarction patients, and 20 neurological autoimmune
disease patients. Let-7 family levels were determined by polymerase chain reaction.
A dual luciferase assay was used to test whether let-7c recognized the 3′UTR of RNF213.
Results
The expression level of let-7c in MMD patients is higher than that observed in the
control groups (P < .001). The luciferase assay results indicated that hsa-let-7c could diminish luciferase
activity from a reporter vector containing the 3′-UTR of RNF213 (P < .05). The suppression of luciferase activity is not found in mutRNF213 (P > .05).
Conclusions
Increased expression of let-7c in MMD patients may contribute to MMD pathogenesis
by targeting RNF213. Thus, let-7c may be a potential biomarker for the diagnosis of MMD.
Key Words
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Article info
Publication history
Published online: June 09, 2015
Accepted:
January 27,
2015
Received in revised form:
January 23,
2015
Received:
December 22,
2014
Footnotes
This study was funded by National Natural Science Foundation of China (81371385).
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2015.01.041
Copyright
© 2015 National Stroke Association. Published by Elsevier Inc. All rights reserved.
