Background
Intracerebral hemorrhage (ICH) is a devastating form of stroke and depending on the
underlying cause, primary ICH is mainly caused by hypertension (HTN-ICH) or cerebral
amyloid angiopathy (CAA-ICH). Currently, neuroimaging markers are required to identify
the pattern for each etiology. The discovery of new biomarkers to improve the management
of this pathology is therefore needed.
Methods
A microarray analysis was carried out to analyze gene expression differences in blood
samples from patients (>1.5 months since the last ICH event) who suffered a CAA-ICH
and HTN-ICH, and controls. The results were replicated by quantitative polymerase
chain reaction and the plasma protein level of the best candidate was measured with
enzyme-linked immunosorbent assay.
Results
The microarray analysis and the validation study revealed an increase in Golgin A8
Family, Member A (GOLGA8A) mRNA and protein levels in ICH cases compared to controls
(P < .01), although no differences were found between specific ICH etiologies. GOLGA8A
plasma levels were also associated with the presence of multiple hemorrhages (P < .05).
Conclusions
The GOLGA8A level was increased in the blood of patients who suffered a primary ICH.
We did not, however, find any candidate biomarker that distinguished CAA-ICH from
HTN-ICH. The role of GOLGA8A in this fatal disorder has yet to be determined.
Key Words
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Article info
Publication history
Published online: December 29, 2015
Accepted:
November 22,
2015
Received:
June 3,
2015
Footnotes
Source of funding: The Neurovascular Research Laboratory takes part in the INVICTUS network (RD12/0014/0005). This work was supported by grants from the Fondo de Investigaciones Sanitarias INTRASALUD PI 11/0176, financed by the Institute of Health Carlos III, Spain. C.M.-Z. is supported by the fellowship grant FI12/00089, and M.H.-G., P.D., and I.F.-C. by the Miguel Servet programme (CP12/03259, CP09/136, and CP12/03298, respectively) from the Institute of Health Carlos III, Spain.
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2015.11.032
Copyright
© 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.
