Abstract
Objectives
Dysfunction in mitochondrial activity may have profound role in ischemic stroke-induced
neuronal death, hence maintaining the mitochondrial function seems to be valuable
for neuronal viability and neurological improvement.
Methods
C57BL/6J mice were allocated into sham and stroke groups. Mice in the stroke groups
underwent photothrombosis-induced stroke in the medial prefrontal cortex (mPFC) and
were divided into the following subgroups; RB, Mito 85, Mito 170, and Mito 340, and
received their respective treatments via intra-nasal route every other day (3 days
per week) for one week. A battery of behavioral tests including social interaction,
passive avoidance, and the Lashley III maze was used to investigate social, contextual,
and spatial memories. Moreover, changes in mitochondrial function, including reactive
oxygen species (ROS) and ATP levels, and mitochondrial membrane potential, were assessed
in mPFC. The expression of growth-associated protein 43 (GAP-43), post-synaptic density-95
(PSD-95), and synaptophysin (SYP) was detected by western blotting.
Results
Behavioral results revealed that mitotherapy alleviated ischemia-induced memory impairment.
Also, transplantation of exogenous mitochondria lowered ROS, restored ATP generation,
and improved mitochondrial membrane potential. Induction of ischemia decreased the
levels of synaptic markers in mPFC while exogenous mitochondria (170 and 340µg) significantly
upregulated the expression of GAP-43 and PSD-95 after ischemic stroke.
Conclusion
Our research highlighted the importance of mitotherapy in regulating synaptic markers
expression and mitochondria function, which could represent a potential strategy for
improving cognitive and memory deficits following stroke.
Keywords
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Article info
Publication history
Published online: October 16, 2022
Accepted:
September 19,
2022
Received in revised form:
September 14,
2022
Received:
July 21,
2022
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2022.106801
Copyright
© 2022 Elsevier Inc. All rights reserved.
