Abstract
Objectives
Recent experimental studies have defined intracranial aneurysms as a macrophage-mediated
chronic inflammatory disease affecting intracranial arteries. Although there are various
subtypes in macrophages, what type of macrophages is present in lesions during the
disease development remains to be elucidated.
Methods
The previously-established aneurysm model of rats was used. Macrophages were labeled
with the fluorescent protein and isolated by a laser-microdissection method. The comprehensive
gene expression profile analyses and gene ontology analyses was then done to identify
a macrophage subtype present in lesions at the growth phase.
Results
The gene expression profile data of total 52 macrophages infiltrating into the lesions
was acquired. The principal component analysis revealed the monotonous macrophage
subtype. By comparing the profile identified with one from in vitro-differentiated
M0 or M1 macrophages, the macrophages in the lesions were belonged to the simple and
unique subtype. Because the perception of signaling from nervous system was highlighted
as up-represented terms through gene ontology analyses, the macrophage subtype in
lesions at the growth phase might be differentiated under the influence of nervous
system in the microenvironment. The histopathological examinations supported the above
notion by confirming the presence of nerves in the adventitia.
Conclusions
The findings from the present study have provided the useful insights about the macrophage
subtype in aneurysm lesions at the growth phase and also proposed its ability as a
therapeutic target.
Key Words
Abbreviations:
CCA (common carotid artery), GO (gene ontology), IA (intracranial aneurysm), SAH (subarachnoid hemorrhage)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: October 29, 2022
Accepted:
October 18,
2022
Received in revised form:
October 5,
2022
Received:
August 17,
2022
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2022.106848
Copyright
© 2022 Elsevier Inc. All rights reserved.
