Abstract
Objectives
Ischemic stroke causes high morbidity, mortality and health burden in the world. MiR-342-5p
was associated with Alzheimer's disease and cardio-protection. Herein, we aimed to
reveal effects of miR-342-5p on cerebral ischemia injury as well as novel targets
for stroke.
Materials and methods
AgomiR-342-5p was intracerebroventricularly injected into the middle cerebral artery
occlusion (MCAO) mouse models to evaluate functions of miR-342-5p on cerebral ischemia.
RT-qPCR and western blot assays were used to evaluate genes expression. Oxygen-glucose
deprivation (OGD) was used as an in vitro model for ischemia. Viability and apoptosis ratio of neurons was evaluated by CCK-8,
LDH release detection, and flow cytometry. The potential targets of miR-342-5p were
predicted by Targetscan, and their interaction was confirmed by luciferase assay.
Results
The intervention of miR-342-5p effectively attenuated ischemic injury in MCAO mice.
MiR-342-5p overexpression could protect neurons against OGD-induced injury, as revealed
by increased cell viability and BCL2 expression, and decreased LDH release, apoptosis
ratio, and BAX expression in OGD-induced neurons. Mechanically, miR-342-5p could directly
bound with CCAR2 to inhibit its expression. Overexpressing CARR2 aggravated the OGD-induced
injury of neurons, which was partly restrained by overexpressing miR-342-5p reversed.
Furthermore, miR-342-5p/CARR2 axis regulates Akt/NF-κB signaling pathway in vitro as well as in vivo cerebral ischemia models.
Conclusions
MiR-342-5p inhibited neuron apoptosis by regulating Akt/NF-kB signaling pathway via
CCAR2 suppression. Our findings revealed the neuroprotection of miR-342-5p in cerebral
ischemia.
Keywords
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Article info
Publication history
Published online: November 23, 2022
Accepted:
November 16,
2022
Received in revised form:
October 24,
2022
Received:
August 11,
2022
Identification
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2022.106901
Copyright
© 2022 Elsevier Inc. All rights reserved.
